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Particulate systems and polymers for in vitro and in vivo delivery of polynucleotides

Identifieur interne : 003019 ( Main/Exploration ); précédent : 003018; suivant : 003020

Particulate systems and polymers for in vitro and in vivo delivery of polynucleotides

Auteurs : Philip L. Felgner [États-Unis]

Source :

RBID : ISTEX:C8B5BBA054FE5F93A01083126239B9F0D45157A4

English descriptors

Abstract

Abstract: During the last three decades steady progress has been made towards improving delivery of functional polynucleotides in vitro and in vivo. Laboratory methods for tissue culture cells have advanced to the stage where optimized commercial products for diethylaminoethyldextran, calcium-phosphate- and cationic-lipid-mediated transfection can be obtained in easy-to-use kits. Under some conditions efficiencies approaching 100% of the cells transfected can be obtained. Improvements in the technologies can be expected to bring further increases in the level of expression obtained, higher efficiency and less toxicity. The cationic lipid technology in particular may find broader applications as protein molecules, such as transcriptional and translational regulators or nuclear localization signals, are cotransfected with relevant gene sequences. Methods for delivering functional non-replicating plasmids in vivo without the use of retroviruses are currently in their infancy; however, the data indicate that direct delivery and in vivo expression of genes are possible. During the next decade new and simplified delivery systems and appropriate therapeutic endpoints will likely be identified to take advantage of endogenous gene expression following direct injection of purified genes in vivo.

Url:
DOI: 10.1016/0169-409X(90)90015-K


Affiliations:


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Le document en format XML

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<term>Didodecyldimethylammonium bromide bilayer vesicles</term>
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<term>Forms liposomes</term>
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<term>Functional polynucleotides</term>
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<term>Fusogenic behavior</term>
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<term>Gene delivery</term>
<term>Gene expression</term>
<term>Gene product</term>
<term>Gene sequences</term>
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<term>Highest levels</term>
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<term>Lipid vesicles</term>
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<term>Negative charge</term>
<term>Neoplastic cells</term>
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<term>Normal protein pattern</term>
<term>Nuclear fraction</term>
<term>Nuclear recognition proteins</term>
<term>Nucleic</term>
<term>Nucleic acids</term>
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<term>Other phospholipids</term>
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<term>Particulate systems</term>
<term>Particulate systemsand polymersfor polynucleotidedelivery</term>
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<term>Pharmaceutical applications</term>
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<term>Phospholipid vesicles</term>
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<term>Plasma membrane</term>
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<div type="abstract" xml:lang="en">Abstract: During the last three decades steady progress has been made towards improving delivery of functional polynucleotides in vitro and in vivo. Laboratory methods for tissue culture cells have advanced to the stage where optimized commercial products for diethylaminoethyldextran, calcium-phosphate- and cationic-lipid-mediated transfection can be obtained in easy-to-use kits. Under some conditions efficiencies approaching 100% of the cells transfected can be obtained. Improvements in the technologies can be expected to bring further increases in the level of expression obtained, higher efficiency and less toxicity. The cationic lipid technology in particular may find broader applications as protein molecules, such as transcriptional and translational regulators or nuclear localization signals, are cotransfected with relevant gene sequences. Methods for delivering functional non-replicating plasmids in vivo without the use of retroviruses are currently in their infancy; however, the data indicate that direct delivery and in vivo expression of genes are possible. During the next decade new and simplified delivery systems and appropriate therapeutic endpoints will likely be identified to take advantage of endogenous gene expression following direct injection of purified genes in vivo.</div>
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